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. 2020 Mar 21;125(6):905–923. doi: 10.1093/aob/mcaa022

Fig. 8.

Fig. 8.

Immunolocalization of trans-zeatin riboside in 2-week-old nodules of pea Sprint-2 wild type (A–C), corresponding sym31 mutant (D–F) and sym26 mutant (G–L). (A–C) Infected cell from the nitrogen fixation zone. (D–F) Infected cell from the zone corresponding to the nitrogen fixation zone. (G–I) Recently infected cell with an infection thread and droplet. (J–L) Infected cell from the zone corresponding to the nitrogen fixation zone. (A, D, G, J) Merge of differential interference contrast (DIC) and magenta channel. (B, E, H, K) Merge of green and magenta channels. A single optical section is presented: trans-zeatin riboside in green and DNA (bacteria and nuclei) in magenta. (C, F, I, L) The heatmap shows colour-coded fluorescence signal intensities for the green signal channel; the quantification scale is the same for all images. PI, propidium iodide; AF488, Alexa Fluor 488. n, nucleus; arrowheads indicate infection droplets. Scale bars are 10 µm.