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. Author manuscript; available in PMC: 2020 May 13.
Published in final edited form as: Stem Cell Res. 2020 Feb 19;44:101737. doi: 10.1016/j.scr.2020.101737

Fig. 1.

Fig. 1.

Characterization of TRNDi001-D iPSC line. A) Left: phase contrast imaging of TRNDi001-D colonies grown on Matrigel. Right: Representative immunofluorescent images of iPSCs positive for stem cell markers: SOX2, OCT4, NANOG, and SSEA4. Nucleus is labelled with Hoechst 33342 (blue). B) Flow cytometry analysis of pluripotency protein markers: TRA-1–60, NANOG, and SSEA4. C) Cytogenetic analysis showing a normal karyotype (46, XY). D) Detection of homozygous gene mutation of p. I1061T (c.3182T>C) in exon 21 of the NPC1 gene. E) RT-PCR verification for the clearance of the Sendai virus from reprogrammed cells. Sendai virus vector transduced fibroblasts were used as a positive control. F) Pathological analysis of teratoma from TRNDi001-D iPSC, showing a normal ectodermal, mesodermal, and endodermal differentiation.