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. Author manuscript; available in PMC: 2020 May 13.
Published in final edited form as: Stem Cell Res. 2020 Feb 19;44:101737. doi: 10.1016/j.scr.2020.101737

Table 2.

Reagents details.

Antibodies used for immunocytochemistry/flow-cytometry Antibody Dilution Company Cat # and RRID
Pluripotency Markers Mouse anti-SOX2 1:50 R & D systems, Cat# MAB2018, RRID: AB_358009
Pluripotency Markers Rabbit anti-NANOG 1:400 Cell Signaling Technology, Cat# 4903, RRID: AB_10559205
Pluripotency Markers Rabbit anti-OCT4 1:400 Thermo Fisher Scientific, Cat# A13998, RRID: AB_2534182
Pluripotency Markers Mouse anti-SSEA4 1:1000 Cell Signaling Technology, Cat# 4755, RRID: AB_1264259
Secondary Antibodies Donkey anti-Mouse IgG (Alexa Fluor 488) 1:400 Thermo Fisher Scientific, Cat# A21202, RRID: AB_141607
Secondary Antibodies Donkey anti-Rabbit IgG (Alexa Fluor 594) 1:400 Thermo Fisher Scientific, Cat# A21207, RRID: AB_141637
Flow Cytometry Antibodies Anti-Tra-1–60-DyLight 488 1:50 Thermo Fisher Scientific, Cat# MA1–023-D488X, RRID: AB_2536700
Flow Cytometry Antibodies Anti-Nanog-Alexa Fluor 488 1:50 Millipore, Cat# FCABS352A4, RRID: AB_10807973
Flow Cytometry Antibodies anti-SSEA-4-Alexa Fluor 488 1:50 Thermo Fisher Scientific, Cat# 53-8843-41, RRID: AB_10597752
Flow Cytometry Antibodies Mouse-IgM-DyLight 488 1:50 Thermo Fisher Scientific, Cat# MA1–194-D488, RRID: AB_2536969
Flow Cytometry Antibodies Rabbit IgG-Alexa Fluor 488 1:50 Cell Signaling Technology, Cat# 4340S, RRID: AB_10694568
Flow Cytometry Antibodies Mouse IgG3-FITC 1:50 Thermo Fisher Scientific, Cat# 11-4742-42, RRID: AB_2043894
Primers Target Forward/Reverse primer (5’–3’)
Sev specific primers (RT-PCR) Sev/181 bp GGA TCA CTA GGT GAT ATC GAG C/ ACC AGA CAA GAG TTT AAG AGA TAT GTA TC
Sev specific primers (RT-PCR) KOS/528 bp ATG CAC CGC TAC GAC GTG AGC GC/ ACC TTG ACA ATC CTG ATG TGG
Sev specific primers (RT-PCR) Klf4/410 bp TTC CTG CAT GCC AGA GGA GCC C/ AAT GTA TCG AAG GTG CTC AA
Sev specific primers (RT-PCR) C-Myc/523 bp TAA CTG ACT AGC AGG CTT GTC G/ TCC ACA TAC AGT CCT GGA TGA TG
House-Keeping gene (RT-PCR) GAPDH/197 bp GGA GCG AGA TCC CTC CAA AAT/ GGC TGT CAT ACT TCT CAT GG
Targeted mutation analysis (PCR) NPC1/321 bp TCC AGG GTC AGG TGA TTT TGC/ TGA AAA AGG GCA GGC TTT ACC