KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| CAIX | Abcam | 2D3, RRID:AB_2066533 |
| SMAD2/3 | BD Biosciences | RUO; RRID:AB_398161 |
| SMAD2/3 phospho-Ser465/467 | CST | D6G10; RRID:AB_2631089 |
| HIF1α | CST | D2U3T; RRID:AB_2622225 |
| HIF2α | Novus Biologicals | NB100; RRID:AB_10002593 |
| TGFBR2 | Abcam | EPR14673; RRID:AB_2818975 |
| Akt | CST | 9272; RRID:AB_329827 |
| Akt phosphor-Ser473 | CST | D9E; RRID:AB_2315049 |
| SUCLG2 | ThermoFisher Scientific | PA5-61289; RRID:AB_2648068 |
| Vinculin | Sigma | VIN115; RRID:AB_477617 |
| GAPDH | CST | 14C10; RRID:AB_561053 |
| β-Actin | Sigma | AC15; RRID:AB_476744 |
| Anti-Mouse IgG HRP-linked secondary | CST | 7076; RRID:AB_330924 |
| Anti-Rabbit IgG HRP-linked secondary | CST | 7074; RRID:AB_2099233 |
| Biological Samples | ||
| Primary omental tissue (for ex vivo studies, and isolation of NOF/HPMC) | University of Chicago Department of Obstetrics and Gynecology | https://www.uchicagomedicine.org/conditions-services/obgyn |
| Ovarian cancer tissue micro-array | University of Chicago Ovarian Cancer Research Laboratory | https://obgyn.uchicago.edu/research/lengyel-ovarian-cancer-laboratory |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Low glucose (5mM) DMEM | Life Technologies | 11885-084 |
| DMEM | Corning | 10-013-CV |
| RPMI1640 | Corning | 10-040-CV |
| FB-Essence | VWR | 10803-034 |
| Penicillin/Streptomycin (100X) | Corning | 30-002-CI |
| MEM non-essential amino acids | Corning | 25-025-CI |
| MEM vitamins | Corning | 25-020-CI |
| Metformin | Sigma | D150959 |
| AICAR | Sigma | A9978 |
| Dorsomorphin (Compound C) | Sigma | P5499 |
| N-oxalylglycine | Sigma | O9390 |
| 2-oxoglutarate | Sigma | 349631 |
| Dimethyl succinate | Sigma | W239607 |
| Q5 Site-Directed Mutagenesis Kit | NEB | E0554S |
| Lipofectamine 2000 | ThermoFisher Scientific | 11-668-500 |
| Recombinant human IL-8 | Peprotech | 200-08M |
| Recombinant human CCL2 | Peprotech | 300-04 |
| Recombinant human TGFβ1 | Life Technologies | PHG9204 |
| CXCR1/IL8RA neutralizing antibody | R&D Systems | MAB330-SP |
| LY364947 | Tocris Bioscience | 2718 |
| Critical Commercial Assays | ||
| Quantikine ELISA – murine CXCL1 | R&D Systems | MKC00B |
| Quantikine ELISA – murine CCL2 | R&D Systems | MJE00B |
| Proteome Profiler Mouse Cytokine Array Kit – Panel A | R&D Systems | ARY006 |
| Seahorse XF96 Cell Mito Stress Test | Seahorse Bioscience | 103015-100 |
| Experimental Models: Cell Lines | ||
| TYKnu | University of California Los Angeles | Koneczny Laboratory |
| HeyA8 | MD Anderson Cancer Center | Mills Laboratory |
| DOV13 | University of Illinois at Chicago | Burdette Laboratory |
| Experimental Models: Organisms/Strains | ||
| C57BL/6NCrl Inbred Mice | Charles River | 027 |
| Oligonucleotides | ||
| Please see Table S6 for oligonucleotide sequences. | ||
| Recombinant DNA | ||
| HA-HIF1α P402A/P564A-pcDNA3 | Yan et al., 2007 | Addgene: 338299 |
| pH IV-ODD-EGFP-IRES-dTomato | Bagnall et al., 2014 | Addgene: 395015 |
| SUCLG2 | DNASU | HsCD00445557 |
| CXCR1 | Sino Biological | HG10856-NF |
| CCR2 | Sino Biological | HG16084-NF |
| EF1a-GFP-IRES-Puro (pEGIP) | Zou et al., 2009 | Addgene: 26777 |
| Software and Algorithms | ||
| GraphPad Prism 7 | GraphPad Software | RRID: SCR_002798 |
| FIJI [ImageJ] | National Institutes of Health | RRID:SCR_002285 |
| Aperio ScanScope / Pixel Count v9 | Leica Biosystems | RRID: SCR_014311 |
| Thermo Xcalibur | ThermoFisher Scientific | RRID: SCR_014593 |
| MaxQuant | Max Planck Institute | RRID: SCR_014485 |
| Perseus | Max Planck Institute | RRID: SCR_015753 |
| Molecular Signatures Database | Broad Institute, MIT | RRID: SCR_016863 |
| MetaboAnalyst | McGill University | RRID: SCR_015539 |
| Other | ||
| Invasion transwell insert (8μm pore) | ThermoFisher Scientific | 353097 |
| Co-culture transwell insert (0.4μm pore) | Thomas Scientific | 3450 |
| Collagen type 1 [rat tail] | Corning | 354236 |
| PureLink HiPure Plasmid Maxiprep Kit | ThermoFisher Scientific | K2100-06 |
| IDEXX CellCheck | IDEXX Bioresearch | https://www.idexxbioanalytics.com/cellcheck |