Figure 1.

Flavonoids in A. conyzoides inhibited the proliferation, invasion, migration, and clonality of HeLa cells in vitro. (a) Effects of gradient concentrations of flavonoids (50-500 μg/mL) on the cell survival rate of HeLa cells were tested using the MTT assay (^∗∗P < 0.01 and ^∗∗∗P < 0.001, compared to control). After treatments with increasing doses of flavonoids (50, 200, and 400 μg/mL) for 48 h, the invasion ability of HeLa cells was tested by the wound healing assay (b), and the migration ability (c, e) and clonality (d, f) of HeLa cells were investigated using the transwell and clonogenic assays, respectively (^∗∗∗P < 0.001, compared to control).