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. 2019 Aug 9;30(3):1318–1329. doi: 10.1093/cercor/bhz168

Figure 4.

Figure 4

Elimination of Cdh2 from postmitotic precursors decreases calretinin and somatostatin interneuron cell numbers in the adult primer somatosensory cortex. (A, B) Calretinin (Calb2) in situ hybridization in +/+ (A) and −/− (B) animals. (C) Statistical analysis of Calb2-positive cell density shows significant difference between littermates. Two-tailed unpaired t-test, P < 0.0001; +/+: n = 7; −/−: n = 7; graph shows raw data points and means ± SEM. (D, E) Somatostatin (Sst) in situ hybridization in animal pairs. (F) Statistical analysis of Sst-positive cell density shows significant difference between +/+ and −/−. Mann–Whitney U test, P = 0.0041; +/+: n = 11; −/−: n = 11. Graph shows raw data points and medians ± interquartile range (IQR). (G, H) Sst and Calb2 double immunostaining. (I, J) Statistical analysis demonstrates a significant decrease in both Calb2-positive and Calb2/Sst double-positive cell density in −/− animals. Mann–Whitney U test, Calb2 P < 0.0001; Calb2/Sst P = 0.0024; +/+: n = 6; −/−: n = 6. Statistical figures show raw data points and medians ± interquartile range (IQR). n indicates the number of mice per group. Scale bars indicate 100 μm.