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. 2020 May 7;3(6):e201900545. doi: 10.26508/lsa.201900545

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© 2020 Ngan et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Figure S4. — Cumulative phospho-ErbB3(Y1289)/total-ErbB3 ratio of MAPK-mutants expressing as well as GDC-0994 inhibited FaDu cells. (A) Cumulative quantification plots of phospho-ErbB3(Y1289)/total-ErbB3 ratio upon ectopic expression of mutants and WTs of MAPK1, ARAF, BRAF, HRAS, MAP2K1, and MAP2K2 in FaDu cells (N ≥ 4 independent experiments). (B) Cumulative quantification plots of phospho-ErbB3(Y1289) and total-ErbB3 levels in HNSCC Pt.25 primary tumor cultures (carrying both HRAS p.G12S and MAPK1 p.R135K mutations), HSC-6 cell line (carrying MAPK1 p.E322K mutation) and HSC4 (MAPK pathway WT, CCLE) upon MAPK inhibitor GDC-0994 treatment for 30 min. The quantified changes of p-ErbB3(Y1289)/total-ErbB3 ratio upon GDC-0994 treatment were showed (N ≥ 4 independent experiments).