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. 2020 May 6;11:604. doi: 10.3389/fphar.2020.00604

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Copyright © 2020 Zheng, Chen, Wang, Huang, Liu, Yang, Zhou, Zhao, Luo, Li and Chen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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qPCR validation of the identified keystone microbiome markers. (A) Pearson correlation between relative abundance of the genera Methanobrevibacter, Enterococcus and Blautia from 16S rDNA sequencing and qPCR data. Colors and shapes of the plot symbols indicate samples from different treatment groups. Shaded area represents the 95% confidence interval for the regression line. (B) Relative abundance of the genera Methanobrevibacter, Enterococcus, and Blautia across treatment groups, as detected by qPCR. Variations between groups were tested by Wilcoxon rank-sum test with FDR correction. Only significant differences between groups (p < 0.05) were annotated. FDR, false discovery rate.