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. 2020 May 13;40(20):3896–3914. doi: 10.1523/JNEUROSCI.0102-20.2020

Figure 3.

Figure 3.

Co-labeling AAV-mSncg-EGFP infected hPSC-derived human RGCs with RGC reporter Brn3b-tdTomato. A, Confocal images of cultured hPSC-RGCs showing EGFP+ and tdTomato+ RGCs. Scale bar, 20 µm. B, Quantification of EGFP/tdTomato double-positive cells. Data are presented as mean ± SEM, n = 12; ****p < 0.0001; Student's t test. C, Epi-fluorescence images of human primary RGCs infected with AAV-mSncg-EGFP or AAV-hSncg-EGFP at the same ratio. D, Epi-fluorescence images of human stem cell-derived RGCs infected with AAV-mSncg-EGFP or AAV-hSncg-EGFP at different ratio. Scale bar, 50 µm.