| Temperature | Time |
|---|---|
| 18° C | 10 min; in addition to the 5 min at 18° C in step xiii |
| 25° C | 10 min |
| 37° C | 30 min |
| 42° C | 10 min |
| 70° C | 20 min |
| 4° C | Until removed from thermal cycler |
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