The INTACT method for cell type-specific gene expression and chromatin profiling in Arabidopsis

. Author manuscript; available in PMC: 2020 May 13.

Published in final edited form as: Nat Protoc. 2010 Dec 16;6(1):56–68. doi: 10.1038/nprot.2010.175

Step number	Denature	Anneal/Extend	Hold
1	94° C, 2 min
2–18	94° C, 30 sec	70° C, 5 min
19			4° C, until removed from thermal cycler