| Buffer | Volume | Time |
|---|---|---|
| 1. Low salt wash | 0.5 ml | 5 min |
| 2. High salt wash | 0.5 ml | 5 min |
| 3. LiCl wash | 0.5 ml | 5 min |
| 4. TE | 0.5 ml | 5 min |
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