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Increased cytotoxicity of BaxS184del is due to enhanced apoptosis. HeLa cells were transfected with indicated bax constructs or vector control. The cells were collected 24 h after transfection and analyzed for apoptosis. Apoptosis was determined either as (a) mitochondrial depolarization using mitochondrial potential sensitive dye DiOC6 or as (b) phosphatidyl serine exposure by Annexin V staining. Data are mean ± s.d. of triplicate observations (*P<0.01).
