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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Mol Cancer Res. 2019 Dec 20;18(4):574–584. doi: 10.1158/1541-7786.MCR-19-0657

Figure 4: MGA acts as a repressor for cancer cell proliferation.

Figure 4:

A. siRNA-mediated silencing of MYC (verified by immunoblots) decreased the proliferation of A549 cells. Cell number was counted five days post transfection and normalized to the negative control siRNA. P values are derived from t tests. Error bars: s.d., **P<0.01.

B. Same experiment as A, but in NCI-H23 cells, Error bars: s.d., ***P<0.001.

C. Schematic chart explaining the design of competitive cell proliferation assays. A549 and NCI-H23 cells were first transfected with empty-ZsGreen control or MGA-ZsGreen vector. The transfected cells were collected based on GFP signal and mixed with parental cells at 1:1 ratio. The percentage of GFP-positive cells was then counted over time.

D. MGA overexpression (verified by immunoblots) decreased the proliferation of A549 cells. The percentage of GFP-positive cells was counted four and six days post seeding the cells and normalized to the empty-ZsGreen control. P values are derived from t tests. Error bars: s.d., *P<0.05; ***P<0.001.

E. Same experiment as D, but in NCI-H23 cells, Error bars: s.d., **P<0.01; ***P<0.001.