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. 2020 May 6;11:736. doi: 10.3389/fimmu.2020.00736

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Copyright © 2020 Bautista, Vásquez, Ayala-Ramírez, Téllez-Sosa, Godoy-Lozano, Martínez-Barnetche, Franco and Angel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Light chain repertoire profile of B cells subsets. Frequencies of IGKV family (A), IGKJ genes (B) and IGKV genes (C) used by different B cells subsets are shown. Samples from three donors were pooled and analyzed with Fisher’s test with p-value correction using FDR. and IGKV region mutations frequencies (D) were analyzed by Kruskal-Wallis test using Dunn’s multiple comparison test correction. The median and interquartile range of the three donors is shown. Only statistically significant differences observed in all three donors are shown. **p < 0.005, ***p < 0.0005, ****p < 0.00005, n = 3 IGKV region mutations frequencies depicted as fraction of clones within six different 2% mutation intervals, from 0 to 2% (left) to >10% mutation frequency (far right) (E). meadian of KCDR3 length is shown (F).