TABLE 1.
Frequencies and percentages of clonotypes in IGH.
|
Percentage of shared clonotypes |
|||||
| Group | Number of shared | Naïve | IgMlo | IgMhi | IgM |
| clonotypes | Only | ||||
| IgMhi Naïve | 1 | 0.04 | 0.03 | ||
| IgMlo IgM only IgMhi | 2 | 0.14 | 0.06 | 0.20 | |
| IgMlo IgMhi | 22 | 1.49 | 0.66 | ||
| IgMlo IgM only | 2 | 0.14 | 0.20 | ||
| IgM Only IgMhi | 55 | 1.65 | 5.39 | ||
| Total of shared clonotypes (%) | 166 | 1 (0.04) | 26 (1.76) | 80 (2.39) | 59 (5.78) |
| Total of unique clonotypes (%) | 8447 | 2775 (99.96) | 1449 (98.24) | 3262 (97.61) | 961 (94.22) |
| Total of clonotypes | 8613 | 2776 | 1475 | 3342 | 1020 |
Numbers and percentages of IGH defined clonotypes shared between B cells subsets. Clonally related sequences were identified based on 100% HCDR3 nucleotide identity, and the same usage of IGHV and IGHJ genes. Data from the three donors were pooled and total, unique, and shared clonotypes are shown for each subset. Values above 1% are shown in bold.