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Effect of cwp22 mutation on toxin expression.
A. Determination of toxin concentration in C. difficile supernatants.
B. Determination of toxin expression on transcription level.
C. Determination of intracellular toxin concentration by western blot and histogram analysis. Experiments were independently repeated thrice.
Bars stand for mean ± SEM (*P < 0.05, **P < 0.01). One-way ANOVA was used for statistical significance.
