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. Author manuscript; available in PMC: 2020 May 13.
Published in final edited form as: Environ Microbiol. 2019 Jun 28;21(8):3076–3090. doi: 10.1111/1462-2920.14706

Fig. 3.

Fig. 3.

Effect of cwp22 mutation on cytotoxicity of C. difficile supernatants on CT26 cells. The CT26 cells were exposed to twofold serial dilutions of C. difficile culture supernatants. The cytotoxic titre was defined as the reciprocal of the highest dilution that caused 50% CT26 cells rounding. Purified Tcd B and BHIS media were used as positive and negative controls respectively. Experiments were independently repeated thrice. Bars stand for mean ± SEM (*P < 0.05).One-way ANOVA was used for statistical significance.