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. Author manuscript; available in PMC: 2020 May 13.
Published in final edited form as: Environ Microbiol. 2019 Jun 28;21(8):3076–3090. doi: 10.1111/1462-2920.14706

Fig. 4.

Fig. 4.

Effect of cwp22 mutation on cell wall integrity.

A. Triton X-100 autolysis assay.

B. LDH cytotoxicity assay.

C. Percent of dead cells.

D. Detection of cell viability. Top panel: images from the bright field; middle panel: images from the merged green/red staining with CFDA and PI respectively; bottom panel: images of TEM (80 kv). Experiments were independently repeated thrice. Bars stand for mean ± SEM (*P < 0.05, **P < 0.01). Student’s unpaired t-test was used for two groups comparison. One-way ANOVA was used for comparison of more than two groups..