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. Author manuscript; available in PMC: 2020 May 13.
Published in final edited form as: Environ Microbiol. 2019 Jun 28;21(8):3076–3090. doi: 10.1111/1462-2920.14706

Table 1.

Bacterial strains and plasmids utilized in this study.

Strain or plasmid Genotype or phenotype Reference
E. coli DH5α Cloning host NEB
E. coli HB101 Conjugation donor (Williams et al., 1990)
C. difficile R20291 Clinical isolate; ribotype 027 (Stabler et al., 2009)
R20291::2601 R20291 2601::EMr This work
R20291::2601/pMTL84153 R20291::2601 containing empty plasmid pMTL84153 This work
R20291::2601/pMTL84153-2601 R20291 2601 gene complemented with pMTL84153-2601 This work
R20291/pMTL84153 R20291 containing empty plasmid pMTL84153 This work
Plasmids
pMTL007C-E2 ClosTron plasmid (ColE1, pCD6, catP) (Heap et al., 2010)
pMTL007C-E2-2601/1483a pMTL007C-E2 derivative retargeted to 2601(Cwp22) gene This work
pMTL84151 E. coli-C. difficile shuttle plasmid (pCD6, catP, ColE1 + tra, MCS) (Heap et al., 2009)
pMTL82153 E. coli-C. difficile shuttle plasmid (pCD6, catP, ColE1 + tra, MCS) (Heap et al., 2009)
pMTL84153 E. coli-C. difficile shuttle plasmid (pCD6, catP, ColE1 + tra, Pfdx + MCS) This work
pMTL84153-2601 Complement vector, pMTL84153 containing 2601 gene This work