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. Author manuscript; available in PMC: 2020 May 13.
Published in final edited form as: Sci Signal. 2019 Jul 2;12(588):eaaw9277. doi: 10.1126/scisignal.aaw9277

Figure 3. NEAT1 represses expression of c-Fos mRNA in murine neuronal cells.

Figure 3.

(A) Reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis of the abundance of Neat1 transcript in control or Neat1- targeted siRNA-treated murine N2a cells. Data are n = 5 to 6 independent cultures of N2a cells. ***P < 0.0005 by Student’s t test. (B) RT-qPCR analysis of the abundance of c-Fos mRNA in control or Neat1-targeted siRNA-treated murine N2a cells. Data are cultured cells from n = 3 to 4 experiments; *P < 0.05 by Student’s t-test. (C and D) RT-qPCR analysis of Neat1 transcript (C) and c-Fos mRNA (D) abundance in dorsal CA1 (dCA1) from naïve or contextual fear conditioning (CxFC) trained mice. Data are from n = 8 to 9 mice; *P < 0.05 by Student’s t-test. (E and F) RT-qPCR analysis of Neat1 transcript (E) and c-Fos mRNA (F) abundance in (CxFC) trained mice. Data are cultured primary neurons from n = 4 to 7 experiments; *P < 0.05 by Student’s t-test, ***P < 0.0005 by Student’s t-test.