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. Author manuscript; available in PMC: 2020 May 13.
Published in final edited form as: Sci Signal. 2019 Jul 2;12(588):eaaw9277. doi: 10.1126/scisignal.aaw9277

Figure 5. Neat1 knockdown regulates c-Fos promoter methylation in vivo and improves long-term memory.

Figure 5.

(A) Graphic depiction of siRNA infusion into hippocampal area CA1 and single-pairing contextual fear conditioning paradigm. Briefly, 3- to 7-month-old male C57BL/6 mice were trained 5 days after bilateral infusion of siRNAs and tested 24 hours after training. (B) Freezing behavior of mice described in (A) as a percent of epoch during training phases of the contextual fear conditioning paradigm. No significant difference detected for either the “pre-shock” or “post-shock” epochs. Data are means ± SEM from n = 8 mice, assessed by Student’s t-test. (C) Freezing behavior of mice described in (A) as a percent of total time during the 5-min test trial. Data are means ± SEM from n = 8 mice, *p = 0.0307 by Student’s t-test. (D) ChIP-qPCR analysis of H3K9me2 at the c-Fos promoter in dCA1 tissue from animals sacrificed 5 days after the conclusion of the behavior experiments described in (A). Data are from dCA1 tissue of 4 to 6 mice, *p = 0.0450 by Student’s t-test.