| PCR amplification reaction mix | |
| Component | Volume (uL) |
| 2X SYBR master mix | 7.5 |
| Water | 4.5 |
| 10 uM Primer F | 0.5 |
| 10 uM Primer R | 0.5 |
| Purified cDNA | 2 |
| Total | 15 |
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