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. Author manuscript; available in PMC: 2020 May 13.
Published in final edited form as: Methods Mol Biol. 2019;1933:49–65. doi: 10.1007/978-1-4939-9045-0_3
1. Washing buffer 10–15 min 50 °C
2. Washing buffer 30–60 min 50 °C
3. Washing buffer 30–60 min 50 °C
4. NTE 5 min 37 °C with slowly shaking in incubator
5. NTE 5 min 37 °C with slowly shaking in incubator
6. RNase A 30 min 37 °C with slowly shaking in incubator
7. NTE 5 min room temperature shaking
8. NTE 5 min room temperature shaking
9. Washing buffer 30–60 min 50 °C
10. PBS 5 min room temperature shaking