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. 2020 May 13;15(5):e0232457. doi: 10.1371/journal.pone.0232457

Fig 2. Optimization of temperature and anesthesia for reliable ECG recording in adult zebrafish.

Fig 2

A, Representative electrocardiogram (ECG) for a single fish without anesthesia. Chamber temperature was changed from 22°C to 32°C. B, Heart rate significantly increased with increased chamber temperature, which is independent of anesthesia. Inset shows in-house temperature-controlled chamber. C, Representative ECG for a single fish before and after anesthethia. Temperature was controlled at 28°C. Noise caused by gill movement is reduced after 6 minutes of anesthesia. D, Heart rate to the time after anesthesia. E, Schematic of protocol for conducting ECG with the iWorx system. bpm indicates beats per minute.