Table 2. Median and range of total DNA concentration (ng/μL) and DNA quality (A260/280) produced by 13 extraction protocols tested on five victorian plant pathology herbarium (VPRI) apple powdery mildew specimens.
| Extraction Method | Median (Range) Invitrogen Qubit™ DNA Concentration (ng/μL) | Median (Range) Total DNA concentration Invitrogen Qubit™ (ng) | Median (Range) Agilent 2200 TapeStation® DNA concentration (ng/μL) | Median (Range) Total DNA concentration Agilent 2200 TapeStation® (ng) | Median (Range) NanoDrop 2000™ DNA Quality (A260/280) |
|---|---|---|---|---|---|
| CheX | 0.52 (0–1.25) | 104.4 (0–117.60) | 2.22 (2.11–2.42) | 444 (422–484) | 1.36 |
| InuP | 0.30 (0–0.43) | 60.4 (0–85.40) | 3.67 (2.97–4.18) | 734 (594–836) | 1.29 |
| SDS | 0.11 (0–3.79) | 5.4 (2.75–189.50) | 2.45 (2.07–3.28) | 122.5 (103.5–164) | 2.68 |
| EznS | 13.7 (3.18–26.9) | 1370 (318–2690) | 10.6 (5.2–12.40) | 1060 (520–1240) | 2.17 |
| DnaZ | 0.99 (0.5–1.13) | 49.9 (27.25–56.50) | 2.62 (2.27–3.02) | 131 (113.5–151) | 2.06 |
| EznF | 3.34 (1.33–39.3) | 334 (133–3930) | 3.64 (2.91–9.41) | 364 (291–941) | 1.97 |
| DneP | 0.46 (0.38–3.66) | 46.6 (43.9–366) | 2.49 (1.14–2.66) | 249 (114–266) | 2.05 |
| IspC | 0.94 (0.25–1.93) | 94.3 (24.6–193) | 2.39 (0–3.19) | 239 (0–319) | 1.92 |
| IspS | 0.97 (0.21–2.12) | 96.8 (21–138) | 2.93 (2.31–4.91) | 293 (231–491) | 1.83 |
| WizG | 2.76 (0.74–8.89) | 276 (74–889) | 3.8 (2.82–9.71) | 380 (282–971) | 1.64 |
| EznP | 10.9 (2.82–12.7) | 1090 (282–1270) | 8.89 (3.51–16.60) | 889 (351–1140) | 1.87 |
| CTAB | 0.36 (0–0.55) | 27.2 (0–41.25) | 2.89 (2.19–3.17) | 216.8 (164.3–237.75) | 1.88 |
| DneP+ | 0.36 (0.08–1.84) | 36.3 (8.30–184) | 2.42 (2.04–4.44) | 242 (204–444) | 1.33 |
Extraction method abbreviations: Chelex®100 (CheX), innuPrep Plant DNA (InuP), sodium dodecyl sulphate (SDS), E.Z.N.A.® SP Plant (EznS), DNAzol™ (DnaZ), E.Z.N.A.® Forensic DNA (EznF), Qiagen DNeasy® Plant (DneP), Isolate II Plant DNA Lysis buffer PA1 C (IspC), Isolate II Plant DNA Lysis buffer PA2 S (IspS), Wizard® Genomic DNA Purification (WizG), E.Z.N.A.® Plant (EznP), Cetyl trimethyl ammonium bromide (CTAB) and Qiagen DNeasy® Plant plus PTB (DneP+).