Table 1. Detection and monitoring of CHS alleles associated with DFB resistance in Cx. pipens populations from Italy sampled in 2018.
| Locality | Genotype frequency | Allele frequency (%) | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| N | II | IL | LL | IM | MM | LM | FF | IF | FL | FM | (I) | (L) | (M) | (F) | |
| 1. Bologna (BO) | 30 | 0.60 | 0.20 | 0.03 | 0.14 | 0.03 | -- | -- | -- | -- | -- | 76.6 | 13.3 | 10 | 0 |
| 2. Budrio (BO) | 30 | 0.93 | 0.07 | -- | -- | -- | -- | -- | -- | -- | -- | 96.6 | 3.3 | 0 | 0 |
| 3. Rubizzano (BO) | 28 | 1.0 | -- | -- | -- | -- | -- | -- | -- | -- | -- | 100 | 0 | 0 | 0 |
| 4. Lido delle Nazioni (FE) | 30 | -- | -- | -- | -- | 0.87 | 0.13 | -- | -- | -- | -- | 0 | 6.6 | 93.3 | 0 |
| 5. Lugo (RA) | 30 | 0.70 | 0.20 | -- | 0.03 | -- | -- | -- | -- | -- | 0.07 | 81.6 | 10 | 5 | 3.3 |
| 6. Massa Lombarda (RA) | 27 | 0.30 | 0.11 | 0.59 | -- | -- | -- | -- | -- | -- | -- | 35.1 | 64.8 | 0 | 0 |
| 7. Forlì (FC) | 30 | 0.17 | 0.23 | -- | 0.23 | 0.13 | 0.17 | -- | -- | 0.07 | -- | 40 | 23.3 | 33.3 | 3.3 |
| 8. Bertinoro (FC) | 30 | 0.46 | 0.30 | -- | 0.14 | -- | 0.10 | -- | -- | -- | -- | 68.3 | 20 | 11.6 | 0 |
| 9. Cesena (FC) | 30 | 0.23 | 0.37 | 0.07 | 0.03 | 0.03 | 0.07 | -- | 0.03 | -- | 0.17 | 45 | 28.3 | 16.6 | 10 |
| 10. Santarcangelo di Romagna (RN) | 30 | 0.30 | 0.23 | 0.03 | 0.37 | -- | 0.07 | -- | -- | -- | -- | 60 | 18.3 | 21.6 | 0 |
| 11. Rimini (RN) | 30 | 0.12 | 0.03 | -- | 0.30 | 0.17 | 0.35 | -- | -- | 0.03 | -- | 28.3 | 21.6 | 48.3 | 1.6 |
| 12. Riccione (RN) | 30 | -- | 0.10 | 0.17 | 0.03 | 0.07 | 0.56 | -- | -- | 0.07 | -- | 6.6 | 53.3 | 36.6 | 3.3 |
Frequency of each CHS-1043 genotype in the Italian populations analysed and the corresponding allele frequencies. The number given to each locality matches the number on Fig 1. N = total number of individuals analysed. I = wild susceptible allele I1043; L = resistant allele I1043L; M = resistant allele I1043M; F = resistant allele I1043F. The abbreviation in brackets refers to province. BO: Bologna, FE: Ferrara; RA: Ravenna, FC: Forlì-Cesena, RN: Riccione