Fig 7. Wnt5 and Vang could function independently to regulate DA1/VA1d glomerular rotation.

(A-C) Frontal views of adult ALs from animals expressing UAS-mCD8::GFP under the control of Mz19-Gal4 stained with antibodies against Brp (magenta) and CD8 (green) to visualize the glomerular pattern and the DA1/VA1d dendrites respectively, in the Vang⁶ (A), Wnt5⁴⁰⁰ (B) and Wnt5⁴⁰⁰; Vang⁶ (C) mutants. (D) Quantification of the DA1/VA1d angles in panels A and C showing that the rotational defect is stronger in the Wnt5⁴⁰⁰; Vang⁶ mutant than in the Vang⁶ mutant. (E) Quantification of the DA1/VA1d angles in B and C showing that the rotational defect is stronger in the Wnt5⁴⁰⁰; Vang⁶ mutant than in the Wnt5⁴⁰⁰ mutant. (F). Graph summarizing the DA1/VA1d angles in the Vang⁶ and Wnt5⁴⁰⁰ single mutants and Wnt5⁴⁰⁰; Vang⁶ double mutant. Student’s t tests were used to compare the angles of the various mutants. Scale bars: 10 μm.