KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| RhoA | Cytoskeleton, Inc. | Cat# ARH04; RRID: AB_2728698 |
| Myosin Light Chain II | Cell Signaling | Cat# 3672; RRID: AB_10692513 |
| Phospho-Myosin Light Chain II | Cell Signaling | Cat# 3671; RRID: AB_330248 |
| β-Actin | Sigma | Cat# A1978; RRID: AB_476692 |
| β-tubulin | Developmental Studies Hybridoma Bank | Cat# 6G7; RRID: AB_528497 |
| Goat-anti-mouse | Rockland | Cat# 610-731-124; RRID: AB_220145 |
| Goat anti-mouse | Jackson Labs | Cat# 715-036-151; RRID: AB_2340774 |
| CD45 | BioLegend | Cat# 103110; RRID: AB_312975 |
| Bacterial and Virus Strains | ||
| BL21(DE3)pLysS | Life Technologies | C6060-03 |
| DH5 alpha | Life Technologies | 18265-017 |
| Biological Samples | ||
| PDX Sample 1-metastatic | UI Tissue Procurement Core | N/A |
| PDX Sample 4-metastatic | UI Tissue Procurement Core | N/A |
| PDX Sample 8-primary | UI Tissue Procurement Core | N/A |
| PDX Sample 16-metastatic | UI Tissue Procurement Core | N/A |
| PDX Sample 17-primary | UI Tissue Procurement Core | N/A |
| PDX Sample 19-primary | UI Tissue Procurement Core | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| (−)-Blebbistatin | Selleckchem | S7099 |
| Fasudil (HA-1077) | Selleckchem | S1573 |
| Staurosporine | Selleckchem | S7099 |
| ML-7 | Sigma-Aldrich | I2764 |
| CK-666 | Millipore Sigma | 182515 |
| SMIFH2 | Millipore Sigma | 344092 |
| Hoechst 33342 | Invitrogen | H1399 |
| Hoechst 33258 | Thermo Fisher | H3569 |
| Propidium Iodide | Invitrogen | P1304MP |
| K+ D-Luciferin | GoldBio | LUCK-1G |
| Ionomycin | Sigma-Aldrich | I9657 |
| DRAQ5 | ThermoFisher | 62251 |
| Lysophosphatidic acid | Sigma | L7260 |
| Critical Commercial Assays | ||
| APC Annexin V | BioLegend | 640920 |
| Cell Titer Blue | Promega | G8080 |
| Phalloidin-AlexaFluor488 | ThermoFisher | A12379 |
| Experimental Models: Cell Lines | ||
| PC-3 | ATCC | CRL-1435 |
| MDA-MB-231 | ATCC | HTB-26 |
| TCCSUP | ATCC | HTB-5 |
| Primary Bladder Epithelial Cells | ATCC | PCS-420-010 |
| MCF-10A | ATCC | CRL-10317 |
| PrEC-LH | Dr. William Hahn | PMID: 15604246 |
| GS689.Li | Our Lab | (Drake et al., 2009) |
| Experimental Models: Organisms/Strains | ||
| NSG.Cg-PrkdcscidIl2rgtm1Wjl/SzJ | The Jackson Laboratory | 005557 |
| NOD-Prkdcem26Cd52Il2rgem26Cd22/NjuCrl | Charles River | 572 |
| NCI BALB/cAnNcr | Charles River | 555 |
| C57BL/6J-Tyrc-2J/J ROSA26LSL-Luc;Ptenfl/fl;Pb-Cre4+ | Our Lab | (Svensson et al., 2011) |
| B6.129P2-Trp53tm1Brn/J | The Jackson Laboratory | 008462 |
| Recombinant DNA | ||
| pLKO.1_RhoA shRNA | Sigma-Aldrich | TRCN0000047712 |
| pLKO.1_RhoA shRNA | Sigma-Aldrich | TRCN0000047711 |
| pLKO.1_SCR shRNA | Sigma-Aldrich | SHC001 |
| pQCXIN-luc | Our Lab | (Drake et al., 2005) |
| pGEX_RBD | Dr. Kris DeMali | (Varzavand et al., 2016) |
| pQCXIP-EGFP | Dr. Christopher Stipp | (Varzavand et al., 2016) |
| Other | ||
| FITC Dextran (3000 MW) | LifeTechnologies | D1821 |
| FITC Dextran (10000 MW) | LifeTechnologies | D3305 |
| FITC Dextran (40000 MW) | LifeTechnologies | D1844 |
| FITC Dextran (70000 MW) | LifeTechnologies | D1823 |
| 15μm Scarlet Microspheres | Invitrogen | F8843 |
| 2μm YG microspheres | Polyscience | 17155-2 |
| PHD1000 Syringe Pump | Harvard ADDaratus | 703006 |