Table 1. Crystallographic data collection and refinement statistics.

	Rab8A:GDP	Rab8A:GppNHp
Crystal parameters
Space group	C2	P21
Cell constants	a = 117.6 Å; b = 75.2 Å; c = 107.7 Å; β = 100.5 Å	a = 34.9 Å; b = 93.7 Å; c = 56.1 Å; β = 103.0 Å
Subunits/AU1	5	2
Data collection
Beam line	X06SA, SLS	X06SA, SLS
Wavelength (Å)	1.0	1.0
Resolution range (Å)2	50–2.4 (2.5–2.4)	50–2.5 (2.6–2.5)
No. observed reflections	105 949	35 644
No. unique reflections3	35 267	11 734
Completeness (%)2	97.2 (96.9)	95.9 (92.3)
Rmerge (%)2,4	0.096 (0.574)	0.123 (0.578)
I/σ (I)2	9.5 (2.1)	6.5 (2.0)
Refinement (REFMAC5)
Resolution range (Å)	30–2.4	30–2.5
No. refl. working set	33 488	11 143
No. refl. test set	1763	586
No. non hydrogen	6875	2845
No. of pSer111	5	2
No. of Mg2+	5	2
No. of nucleotide	5	2
Solvent	45	17
Rwork/Rfree (%)5	23.3/26.2	24.3/27.8
r.m.s.d. bond (Å)/(°)6	0.002/1.2	0.002/1.2
Average B-factor (Å2)	48.4	51.8
pSer111	89.7	55.1
Nucleotide	40.2	43.4
Ramachandran plot (%)7	97.8/2.2/0	98.5/1.5/0
PDB accession code	6STF	6STG

¹Asymmetric unit;

²The values in parentheses for resolution range, completeness, R_merge and I/σ(I) correspond to the highest resolution shell;

³Data reduction was carried out with XDS. Friedel pairs were treated as identical reflections;

⁴R_merge(I) = Σ_hklΣ_j | I(hkl)_j − <I(hkl)> | / Σ_hkl Σ_j I(hkl)_j, where I(hkl)_j is the jth measurement of the intensity of reflection hkl and <I(hkl)> is the average intensity;

⁵R = Σ_hkl | |F_obs| − |F_calc| |/Σ_hkl |F_obs|, where R_free is calculated for a randomly chosen 5% of reflections, which were not used for structure refinement, and R_work is calculated for the remaining reflections;

⁶Deviations from ideal bond lengths/angles;

⁷Percentage of residues in favoured/allowed/outlier region.