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. 2019 Aug 10;11(3):575–587. doi: 10.14336/AD.2019.0810

Figure 2.

Figure 2.

Age-dependent increase in pro-BDNF expression was associated with reduction in sympathetic innervation and mitochondrial activity in eWAT. (A) Immunoblot analysis of BDNF, TH and p-HSL expression in epididymal white adipose tissue (eWAT) of mice at the indicated ages. (n = 4, mean ± S.E.M, **p<0.01, ***p<0.001). (B, C) quantitative PCR analysis. (n = 4, mean ± S.E.M, *p<0.05, **p<0.01, ***p<0.001). (D) Analysis of oxygen consumption rate (OCR) of eWAT obtained from 2, 12 and 18-month old mice with a series of treatments of indicated drugs (oligomycin, carbonyl cyanide-4(trifluoromethoxy)phenylhydrazone (FCCP), and potassium cyanide (KCN)) (n = 3, mean ± S.E.M, *p<0.05, **p<0.01, ***p<0.001). (E) MitoTracker Red CMXRos staining in adipocytes differentiated from PDGFRα+ cells of eWAT of 2, 12, and 18-month-old mice.