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. 2019 Aug 10;11(3):575–587. doi: 10.14336/AD.2019.0810

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Copyright: © 2020 Song et al.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 4. — The major cellular source of pro-BDNF expression in eWAT is PDGFRα⁺ adipocyte progenitors. (A) quantitative PCR analysis of Bdnf expression in adipocytes and PDGFRA⁺ cells isolated from eWAT of mice at the indicated ages (n = 3, mean ± S.E.M, ***p<0.001). Plin1 and Pdgfra expressions were used as specific markers for adipocytes and progenitor cells, respectively. (B) Immunoblot analysis of BDNF expression in adipocytes and PDGFRA⁺ cells isolated from eWAT of mice at the indicated ages (n = 3, mean ± S.E.M, ***p<0.001). Full images of Western blots are shown in supplementary Fig. 4.