Figure 6.

Transfer of esRAGE through BBB. A) In vitro (BBB) model system composed of primary cultures of monkey brain capillary endothelial cells coupled with rat pericytes and astrocytes. Recombinant esRAGE (20 µg/ml) was added to the upper (vessel side) chambers of the model and transferred esRAGE level was quantified in the lower (brain side) chambers. Endothelial cells were treated with scrambled (control) or RAGE shRNA vectors (knockdown). The integrity of the in vitro primate BBB was unaffected by RAGE knockdown, assessed by high trans-endothelial electrical resistance (TEER) (n = 5). B) Human esRAGE levels in brain side were quantified (n = 5). Control, scrambled vector-treated; RAGE knockdown, RAGE shRNA vectors-treated. Values are mean ± SEM.