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. 2020 May 13;6(20):eaaz3559. doi: 10.1126/sciadv.aaz3559

Fig. 5. Proangiogenic B cells in patients with melanoma.

Fig. 5

(A) Frequency of circulating CD73+CD49b+ B cells in patients with melanoma (n = 19) compared to healthy controls (n = 20). (B) Frequency of CD73+CD49b+ B cells in matched PBMC and tumor-derived single-cell suspensions. (C) Expression level of CD39 on CD73−CD49b− and CD73+CD49b+ B cells from PBMCs and tumor tissue. (D) Confocal microscopy staining for CD20, CD138, and Melan A on melanoma tumor tissue. (E) Confocal microscopy staining of CD20, CD49b, CD73, IgG4, and VEGFA on melanoma tumor tissue. Arrows indicate CD138+ plasma cells positive for CD49b, CD73, and VEGFA. (F) Confocal microscopy staining of CD20, CD49b, CD73, IgG4, and CYR61 on melanoma tumor tissue. White arrows indicate CD20+ B cells positive for CD49b, CD73, and IgG4. Yellow arrows indicate CD20+ B cells stained positive for IgG4 and CYR61. (G) Frequencies of tissues stained positive for B cells expressing different markers. The percentage of tissues in which CD20+ or CD138+ cells expressing indicated markers were detected is shown (n = 8).