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. 2020 May 13;6(20):eaay6191. doi: 10.1126/sciadv.aay6191

Fig. 5. Adipocyte-specific inactivation of Zbtb7b diminishes fat-resident Tregs and ILC2s.

Fig. 5

(A) sST2 concentration in CM from explant fat culture and plasma of HFD-fed Flox (n = 12) and ZAKO (n = 9) mice. (B) Representative gating for fat-resident Treg and ILC2 cells from Flox and ZAKO mice. (C) Percentage of immune cells in CD45+ SVF cells from HFD-fed Flox and ZAKO mice. (D) qPCR analysis of eWAT gene expression from Flox and ZAKO mice. Data in (A), (C), and (D) represent means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001; Flox versus ZAKO, two-tailed unpaired Student’s t test. (E) qPCR analysis of gene expression in explant fat culture from HFD-fed Flox and ZAKO mice treated with IL-33 (40 ng/ml) for 24 hours. Data represent means ± SEM (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001, Flox versus ZAKO, two-way ANOVA. Data in (A) to (E) are representative of three independent experiments.