Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 21;69(2):242–249. doi: 10.1538/expanim.19-0137

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

©2020 Japanese Association for Laboratory Animal Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

PMC Copyright notice

Fig. 1. — Generation of c-Maf conditional knockout mice. (A) Schematic targeting strategy for the generation of floxed alleles in the c-Maf locus and the detected genotyping bands for each allele. KI, knock-in; L-HA, left homology arm; R-HA, right homology arm. (B) Genotyping PCR screening of floxed and deleted alleles, including floxed c-Maf (493 bp), Ayu1-Cre (269 bp) and c-Maf deletion (993 bp). (C) HE staining of lens sections from control (c-Maf^fl/fl) and c-Maf^fl/fl; Ayu1-Cre mice at E14.5. The control mice were c-Maf^fl/fl. (D) Immunostaining for c-MAF in lens fiber cells of control (c-Maf^+/+) and c-Maf^fl/fl; Ayu1-Cre mice at E14.5. (E) Genotyping PCR signals for deletion confirmed that c-Maf knockout was successfully induced by tamoxifen injection. All scale bars=100 µm.