Table 1. Sequences of primer pairs used in the real-time quantitative PCR reactions.
Primer Name | Sequence (5’ to 3’) | Accession number | Tm (°C) |
---|---|---|---|
β-actin | CGGAATCCACCAAACCACCTAATCTCCTTCTGCATCCTGTGA | NM_181601.5 | 56 |
SOD | GGCCAACCGATAGTGTTAGACCAGCGTTGCCAGTTTTTAG | NM_131294.1 | 57 |
GPx1a | ACCTGTCCGCGAAACTATTGTGACTGTTGTGCCTCAAAGC | NM_001007281.2 | 58 |
CAT | AGGGCAACTGGGATCTTACATTTATGGGACCAGACCTTGG | XM_021470442.1 | 58 |
sMT-B | TGCTCCAAATCTGGATCTTGGCAGTCCTTCTTGCCCTTAC | NM_001201469.1 | 58 |
MT-2 | AGACTGGAACTTGCAACTGTGGTCAGCTGGAGCCACAGGAATT | NM_001131053.3 | 62 |
Bcl-2 | F:ATGTGCGTGGAAAGCGTCAACR:GAAGGCATCCCAACCTCCATT | NM_001030253.2 | 56 |
p53 | F:GGGCAGGGAGCGTTATGAR:AGAGTCGCTTCTTCCTTCGTC | NM_001271820.1 | 56 |
MDM2 | F:CCGACGCCTCCACTTCTCR:ATAAGGTGCCCAGTCCTTCC | AF010255.1 | 56 |
Bax | F:CGATACGGGCAGTGGCAR:TCGGCTGAAGATTAGAGTTGTT | AF231015.1 | 56 |