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. 2020 Mar 18;98(5):659–671. doi: 10.1007/s00109-020-01894-y

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© The Author(s) 2020

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Fig. 5 — Induction of Cl⁻ secretion by knockdown of PKD1 or PKD2. a, c Original Ussing chamber recordings of polarized M1 cells grown permeable supports (2D culture). Enhanced Cl⁻ secretion by luminal stimulation with ATP (100 μM) or IF (100 μM IBMX and 2 μM forskolin) upon shRNA-knockdown of PKD1 or PKD2. b, d Summaries for calculated equivalent basal short-circuit currents (I_sc) and I_sc activated by ATP and forskolin/IBMX, respectively. e, f Co-staining of TMEM16A (green) and calreticulin. Knockdown of PKD1 or PKD2 upregulated TMEM16A in plasma membrane and cytosol. No colocalization with calreticulin was observed. Bar = 20 μm. Mean ± SEM (number of organoids measured). ^#Significant difference when compared scrambled (p < 0.05; ANOVA)