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. 2020 Mar 18;98(5):659–671. doi: 10.1007/s00109-020-01894-y

Table 1.

RT-PCR primer (mouse)

Tmem16a (mouse) Forward: 5′-GTGACAAGACCTGCAGCTAC 406 bp
Reverse: 5′-GCTGCAGCTGTGGAGATTC
Tmem16a (dog) Forward: 5′-CTATAAGCTCCAGTCCCTAC 513 bp
Reverse: 5′-CGACCCCGTGAATTTTAGTG
Tmem16f (mouse) Forward: 5′-CATACGAATCTAACCTTATCTGC 520 bp
Reverse: 5′-CATTCTCTGTACAGGAGGTAAC
Cftr (mouse) Forward: 5′-GAATCCCCAGCTTATCCACG 544 bp
Reverse: 5′-CTTCACCATCATCTTCCCTAG
αEnac (Scnn1a, mouse) Forward: 5′-CCTTGACCTAGACCTTGACG 409 bp
Reverse: 5′-CGAATTGAGGTTGATGTTGAG
βEnac (Scnn1b, mouse) Forward: 5′-CAATAACACCAACACCCACG 588 bp
Reverse: 5′-GAGAAGATGTTGGTGGCCTG
γEnac (Scnn1g, mouse) Forward: 5′-GCACCGACCATTAAGGACC 464 bp
Reverse: 5′-GCCTTTCCCTTCTCGTTCTC
Nkcc1(Slc12a2, mouse) Forward: 5′-GCGAGAAGGTGCACAATAC 747 bp
Reverse: 5′-CTGTACGGCTCGATCATGTC
Pkd1 (mouse) Forward: 5′-GTGGAAAGCAGGTCGGAAG 236 bp
Reverse: 5′-TCGTCTCGTTCAGCACCAG
Pkd2 (mouse) Forward: 5′-GTGGATGTACACAAGTGAGAAGGAGC 454 bp
Reverse: 5′-CACGACAATCACAACATCCAGACA
Ptch1 (mouse) Forward: 5′-GTCTTGGGGGTTCTCAATGGACTGG 590 bp
Reverse: 5′-ATGGCGGTGGACGTTGGGTTCC
Ptch2 (mouse) Forward: 5′-GTGTGATCCTCACCCCGCTTGACTG 487 bp
Reverse: 5′-CGCTCCAGCCGATGTCATGTGTC
Gapdh (dog, mouse) Forward: 5′-GTATTGGGCGCCTGGTCAC 200 bp
Reverse: 5′-CTCCTGGAAGATGGTGATGG