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. 2020 May 13;10:7928. doi: 10.1038/s41598-020-64916-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of butyric acid on insulin secretion from Min6 cells. (a) Levels of insulin secretion from Min6 cells after treatments with water (H₂O), 100 µmol/L glucose (G), and 100 µmol/L butyric acid (BA) for 24 h were measured by ELISA. (b) Min6 cells pre-treated with Ffar2 or negative control siRNAs before incubation with water, glucose, and butyric acid for 24 h. The level (µg/L) of insulin was detected using a mouse insulin ELISA kit. Data are the mean ± SD from 3 independent experiments. **p < 0.01; ***p < 0.001 vs water treatment and ^#p < 0.05; ^###p < 0.001 vs glucose treatment.