Figure 3.

Antibodies and cytokines produced by infected Sting^−/− mice. C57BL/6 (WT) and Sting^−/− mice were infected with S. mansoni cercariae and on days 0, 7, 19, and 35 after infection total IgG anti-SWAP was measured (n = 5) (a). After 40 days of infection, spleens cells were isolated and stimulated with 200 μg/mL of soluble adult worm antigen (SWAP), medium (negative control) or Concanavalin A (ConA) (positive control). Culture supernatants were tested for IFN-γ (n = 5) (b), IL-4 (n = 5) (c), and IL-10 (n = 5) (d) cytokine measurements. (***) is used to demonstrate statistical differences with p < 0.001 compared to the WT mice. Two-Way ANOVA with Bonferroni adjustments were included for multiple comparisons.