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. 2020 May 13;10:7916. doi: 10.1038/s41598-020-64665-2

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© The Author(s) 2020, corrected publication 2022

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The GFP channel images and the PI channel images are aligned to each other. (A) A GFP coronal slice image from fMOST system for the neuron fiber tracing with 400 μm projection. (B) The corresponding PI image with 10 μm projection. (C) The overlapped image of the white boxes in A and B. (D, E) The raw signals of the white box area in C from the GFP and the PI channel respectively. (F) The overlapped image of D and E indicates the co-localization at single soma resolution. Scale bars: (A,B) 500 μm, (C) 50 μm, (D–F) 10 μm.