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. 2020 May 13;8:20. doi: 10.1038/s41413-020-0096-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — DRG exposed to the secretome of OB-lineage cells have reduced axonal networks. a Scheme of the experimental setup and timeline employed in these experiments. b Representative images of the axonal network of DRG after 48 h exposure to osteogenic medium (OM), and conditioned medium of undifferentiated (MSC CM), immature (D7 OB CM), and mature OB (D21 OB CM) (blue—DAPI; green—βIII-tubulin; dashed circles—DRG core; scale bar—200 µm). Quantification of the area of axonal outgrowth (c) and the core of the ganglion (d) in the different conditions. Results are presented as floating bar graphs (line represents the mean value, and the number above each bar represents the number of ganglia that were analyzed; *P < 0.05, ***P < 0.001, ****P < 0.000 1, ns non-significant)