Fig. 6.

OB-derived Shh contributes to sensory axonal repulsion. a Scheme of the experimental setup and timeline employed in these experiments. b, c Graphical representation of A values (a) and λ values (b) for OB CM alone or supplemented with the Shh activity blocker antibody 5E1, the isotype control antibody anti-BrdU (G3G4), the Smo antagonist SANT-1, and the positive control Shh diluted in OM. Results are presented as floating bar graphs (line represents the mean value, and the number above each bar represents the number of microfluidic devices that were analyzed; **P < 0.01; ns non-significant). d Representative images of the sensory growth cones exposed for 10 min to mature osteoblasts (OB CM) alone, and supplemented with the 5E1 and G3G4 antibodies (green—GAP-43; red—phosphorylated PKCα; scale bar—5 µm). e Graphical representation of the integrated intensity of phosphorylated PKCα in different conditions. Results are presented as violin plot (middle dashed line represents the median value; upper and lower dashed lines represent the quartiles; ****P < 0.000 1, *P < 0.05, ns non-significant)