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. 2020 May 13;10:7938. doi: 10.1038/s41598-020-64660-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Content of 5mC in wheat seedlings quantified by HPLC. (a) 5mC content in leaf of wheat XM13 or wheat AK58, (b) 5mC content in root of wheat XM13 or wheat AK58. CK, T1, T2, T3, T4 or T5 represented the treatment with 0.00, −0.05, −0.15, −0.30, −0.50, −0.75 MPa PEG₆₀₀₀ solution, respectively. The error bar was standard error of mean, the different lowercase letters above bars separately indicated significant difference of DNA methylation among treatments of two wheat cultivars (P < 0.05).