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. 2020 May 13;10:7938. doi: 10.1038/s41598-020-64660-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Methylation status in leaf of wheat seedlings under water deficit. (a,b) represented the ratio of methylation type in leaf of wheat XM13 or wheat AK58, respectively. CK, T1, T2, T3, T4 and T5 indicated wheat seedlings under different osmotic potentials of PEG₆₀₀₀ solution with 0.00, −0.05, −0.15, −0.30, −0.50 or −0.75 MPa, respectively. Type A: methylation monomorphism, ratio of Type A (%) = (band A2 + band A3)/(total methylation bands) ×100; Type B: methylation type, ratio of Type B (%) = (band B1 + band B2 + band B3 + band B4 + band B5)/(total methylation bands) × 100; Type C: demethylation type, ratio of Type C (%) = (band C1 + band C2 + band C3)/(total methylation bands) × 100; Total methylation bands = bands of Type A + bands of Type B + bands of Type C. The error bar was standard error of mean, the different lowercase letters above bars represented the significant difference among treatments of the same methylation type (P < 0.05).