Table 4.
Change of methylation pattern in root of wheat seedlings under water deficit.
| Type of methylation pattern | Number of DNA methylation band | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Wheat XM13 | Wheat AK58 | |||||||||
| CK-T1 | CK-T2 | CK-T3 | CK-T4 | CK-T5 | CK-T1 | CK-T2 | CK-T3 | CK-T4 | CK-T5 | |
| A1 | 195 | 201 | 219 | 228 | 228 | 209 | 211 | 219 | 228 | 231 |
| A2 | 14 | 15 | 15 | 12 | 21 | 13 | 14 | 9 | 18 | 27 |
| A3 | 46 | 49 | 51 | 55 | 56 | 51 | 54 | 52 | 49 | 59 |
| B1 | 0 | 2 | 9 | 4 | 7 | 6 | 9 | 10 | 4 | 9 |
| B2 | 19 | 15 | 17 | 11 | 15 | 17 | 13 | 9 | 13 | 17 |
| B3 | 12 | 18 | 11 | 28 | 23 | 26 | 28 | 27 | 20 | 18 |
| B4 | 0 | 0 | 1 | 3 | 1 | 3 | 5 | 5 | 7 | 9 |
| B5 | 10 | 16 | 19 | 19 | 10 | 13 | 14 | 19 | 22 | 19 |
| C1 | 14 | 16 | 13 | 12 | 12 | 16 | 17 | 9 | 14 | 15 |
| C2 | 1 | 0 | 5 | 1 | 0 | 0 | 0 | 3 | 2 | 0 |
| C3 | 1 | 2 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 5 |
| C4 | 4 | 6 | 10 | 12 | 10 | 12 | 14 | 13 | 9 | 10 |
| Methylation polymorphism (%) | 19.30de | 22.1c | 22.06c | 23.38bc | 20.37d | 25.61ab | 26.77a | 25.53ab | 23.77bc | 24.34b |
The type of DNA methylation pattern was listed as in Table S3, A1-C4 represented change of methylation pattern in root under water deficit as compared to the control (CK). CK, T1, T2, T3, T4 and T5 indicated wheat seedlings under different osmotic potentials of PEG6000 solution with 0.00, −0.05, −0.15, −0.30, −0.50 or −0.75 MPa, respectively. The different lowercase letters in the row indicated the significant difference among treatments of two wheat cultivars (P < 0.05).