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. 2020 May 13;9(5):49. doi: 10.1038/s41389-020-0236-x

Fig. 2. Carbidopa induces apoptosis in LNCaP cells.

Fig. 2

a Flow cytometry analysis of apoptosis in LNCaP cells with increasing doses of Carbidopa. b TUNEL assay in LNCaP cells. Cells were treated with increasing doses of Carbidopa; apoptotic cells were labeled green, and nuclei blue (DAPI). Figure shows merged images. Scale bars = 100 μm. c Quantitative analysis of TUNEL+ cells in at least six separate fields; data are given as means ± SEM. *p < 0.05 versus control. **p < 0.01 versus control. d Cell lysates of LNCaP cells were used to detect the Bax, Bcl-2, and cleaved caspase-3 protein levels by immunoblotting. e Quantitative analysis of each immunoblot; results were normalized to control and data are given as means ± SEM. *p < 0.05 versus control.