Figure 4.

Antibody-Based Validations Confirm Naive-Specific Expression of Cell-Surface Proteins

Histograms of flow cytometry analysis show separation between naive and primed H9 hPSC for several newly identified cell-surface proteins. As a positive control for the assay, CD75 and IL6ST (CD130), which are naive-specific cell-surface markers, and CD57 and CD24, which are primed-specific cell-surface markers were also examined (Collier et al., 2017). Naive H9 hPSC were maintained in t2iLGö on Matrigel-coated plates and primed H9 hPSC maintained in TeSR-E8 on Vitronectin-coated plates. Results are representative of at least three biological replicates. See also Figures S2–S4 and S6.