Figure 1.

Alterations in mRNA Expression of TH Signaling Components during Adult Hippocampal Neurogenesis

Micro-dissected dentate gyri were subjected to FACS and neurogenic/neuronal populations were sorted according to their expression of intracellular markers.

(A) Schematic representation of the hippocampal neurogenic program illustrating expression of stage-specific markers used for sorting and validation strategies.

(B–E) (B) qPCR analysis of neurogenic markers showing that isolated populations are of the expected identity. Relative mRNA expression of (C) TH transporters, (D) TH receptor isoforms, and (E) accessory proteins are depicted. Transcript levels were normalized to Gapdh expression and transcript expression in GCN. Note that, due to their absence from GCN samples, Hes5 and Lat1 values were normalized to NSC levels while Dcx expression was normalized to NB values. n = 2–4 individual samples per cell population. Group means + SEM are shown.